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ADIAVET™ Swine

ADIAVET™ Real time PCR diagnostic kits for swine species

  • Easy-to-use
  • Complete solution
  • Validated processes
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ADIAVET™ diagnostic kits for swine species

By providing quality microbial identification solutions for animal species, we hope to reduce the risk of economic diseases, affecting farmers and breeders. As we also prevent the risk of zoonotic diseases, we contribute to ensuring public health and safety.

Our range of ADIAVET™ ready-to-use PCR kits were designed to make identification of swine diseases easier and faster for you.

From sample preparation…

To perform quality identification, we recommend several DNA/RNA extraction methods adapted to your needs.

For swine species:

  • SILICA MEMBRANE PURIFICATION KITS
  • MAGNETIC BEADS PURIFICATION

…to real time PCR kits : a complete solution for swine species

Our large range of solutions are designed to perform reliable diagnosis of all diseases affecting swine species, including vectorial diseases. These diseases can cause:

  • Reproduction disorders
  • Mobility disorders
  • Nervous system troubles
  • Dead borne pigglets
  • Abortions
  • ...

 

ADIAVET™ kits for Animal Health:

  • APP – Actinobacillus pleuropneumoniae PCR detection kit 

  • BRACHY – Brachyspira PCR detection kit

  • CSFV – Classical Swine Fever PCR detection kit

  • IV – Influenza Virus Type A PCR detection kit for swine host

  • LAW – Lawsonia intracellularis PCR detection kit

  • LEPTO – Leptospira PCR detection kit

  • MYCO HYO – Mycoplasma hyopneumoniae PCR detection kit 

  • PRRS – Porcine reproductive and respiratory syndrome Virus (PRRS) PCR detection kit

  • PRV – Pseudorabies virus (PRV) PCR detection kit, agent of AUJESZKY’S disease

Why choose ADIAVET™ for the detection of veterinary pathogens?

PERFORMANCE : ADIAVET™ processes are validated from sample to identification

FLEXIBILITY : kits include all the different steps of the analysis but remain fully opened for different extraction methods and different thermocyclers

OPTIMIZED WORKFLOW : global extraction method and/or PCR amplification for simultaneous testing of several pathogens

 

DNA/RNA extraction step

In order to achieve very good performances on a wide range of samples, Adiagene always recommends and validates many different DNA/RNA global extraction methods. It is very useful to stay flexible without decreasing any performances (sensitivity, inhibition rate).

PCR amplification step

ADIAVETTM PCR kits are ready-to-use  to reduce risk of mistakes during PCR setup. They are easy-to-use, with only 2 pipetting steps :

  • MasterMix distribution
  • DNA/RNA transfer

They include all components  for DNA amplification. Real Time PCR detection system is based on hydrolysis probe to get a better specificity and to allow multiplexing.

An internal control is included inside each kit to check the quality and stability of the samples, the performances of nucleic acids extraction/purification and the absence of inhibition of the PCR amplification. Positive control(s) included inside each kit are also here to check capability and sensitivity of the kit to detect targeted pathogen(s).

PCR amplification cycles are harmonized as much as possible to give possibility of a multidetection system on the same run and the same thermocycler. Most of Real Time thermocyclers used on the market have been validated by Adiagene and can be used without any risk of performances decrease.

 

ACTINOBACILLUS PLEUROPNEUMONIAE PCR DETECTION KIT

ADIAVET™ APP

Pathogen

Dyes

Samples

Storage

Internal Control

Actinobacillus pleuropneumoniae

Conventional PCR: Single-well multiplex kit

Tissues (lung, tonsil…), colonies on agar

+2/8°C

Exogenous, validates amplification

 

BRACHYSPIRA PCR KIT

ADIAVET™ BRACHY REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Brachyspira hyodysenteriae

Brachyspira pilosicoli

FAM (B. hyodysenteriae)

Cy5 (B. pilosicoli)

VIC (internal control)

Tissues (mucosa), faeces, swabs

+2/8°C

Exogenous, validates amplification

ADIAVET™ BRACHY

Pathogen

Dyes

Samples

Storage

Internal Control

Brachyspira hyodysenteriae

Brachyspira pilosicoli

Brachyspira innocens / Brachyspira intermedia

Single-well multiplex kit

Tissues (mucosa), faeces, swabs

+2/8°C

Exogenous, validates amplification

 

CLASSICAL SWINE FEVER PCR KIT

ADIAVET™   CSF REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Classical Swine Fever Virus

FAM (CSF Virus)

VIC (internal control)

Blood, serum, viral culture, Tissues (spleen, ganglion, tonsils…)

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

INFLUENZA VIRUS TYPE A PCR KIT FOR SWINE HOST

ADIAVET™ SIV REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Swine Influenza A Virus (SIV)

FAM (SIV)

VIC (internal control)

swabs, tissues (lung…), viral culture

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ A/H1N1 (2009) REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Influenza A/H1N1 virus new variant 2009

FAM (A/H1N1)

VIC (internal control)

swabs, tissues (lung…), viral culture

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

LAWSONIA INTRACELLULARIS PCR DETECTION KIT

ADIAVET™ LAW REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Lawsonia intracellularis

FAM (L. intracellularis)

VIC (Internal control)

Tissues (mucosa), faeces, swabs

+2/8°C

Exogenous, validates amplification

 

LEPTOSPIRA PCR KIT

ADIAVET™ LEPTO REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Leptospira interrogans

FAM (Leptospira)

VIC (internal control)

Blood, urine, tissues (kidney, liver, spleen…)

+2/8°C

Exogenous, validates amplification

MYCOPLASMA HYOPNEUMONIAE PCR KIT

ADIAVET™ M. HYOP REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Mycoplasma hyopneumoniae

FAM (Myco. Hyop) VIC (Internal control)

Tisues (lung), tracheobronchial washing, colonies

-20°C +/-5°C

Endogenous (RNase P), validates extraction and amplification

PRRS – PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS PCR KIT

ADIAVET™ PRRS REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Porcine Reproductive and Respiratory Syndrom Virus

FAM (PRRS Virus) VIC (Internal control)

Tissues, swabs, blood,serum, semen

-20°C +/-5°C

Endogenous (RNase P), validates extraction and amplification

ADIAVET™ PRRS EU/NA REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Porcine Reprodutive and Respiratory Syndrom Virus

FAM (PRRS European), Cy5 (PRRS North American), VIC (Internal control)

Tissues (lung, aborted tissues), swabs, blood, serum, semen

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

PSEUDORABIES VIRUS (PRV) PCR KIT AGENT OF AUJESZKY’S DISEASE

ADIAVET™ PRV REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Pseudorabies virus (Aujeszky)

FAM (PRV), VIC (Internal control)

Swabs, tissues (lung, tonsil, brain,...)

-20°C +/-5°C

Exogenous, validates extraction and amplification

 

 

Pioneering diagnostics