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ADIAVET™ Ovine

ADIAVET™ Real time PCR diagnostic kits for ovine species

  • Easy-to-use
  • Complete solution
  • Validated processes
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ADIAVET™ diagnostic kits for ovine species

By providing quality microbial identification solutions for animal species, we hope to reduce the risk of economic diseases, affecting farmers and breeders. As we also prevent the risk of zoonotic diseases, we contribute to ensuring public health and safety.

Our range of ADIAVET™ ready-to-use PCR kits were designed to make identification of ovine diseases easier and faster for you.

From sample preparation…

To perform quality identification, we recommend several DNA/RNA extraction methods adapted to your needs.

For ovine species :

  • DNA EXTRACTION & PURIFICATION KIT ADIAPURE MILK PARATB

…to real time PCR kits : a complete solution for ovine species

Our large range of solutions are designed to perform reliable diagnosis of all diseases affecting bovine species, including vectorial diseases. These diseases can cause:

  • Reproduction disorders
  • Mobility disorders
  • Nervous system troubles
  • Dead borne sheep
  • Abortions

ADIAVET™ kits :

  • BTV – Bluetongue Virus (BTV) PCR detection kit

  • BVD/BD – Bovine Virale Diarrhea PCR detection kit

  • CHLAM – Chlamydophila abortus PCR detection kit

  • COX – Coxiella burnetii PCR detection kit

  • DNA EXTRACTION & PURIFICATION KIT ADIAPURE MILK PARATB

  • LEPTO – Leptospira PCR detection kit

  • SBV – Schmallenberg virus PCR detection kit

TOXO – Toxoplasma gondii GONDII PCR detection kit

Why choose ADIAVET™ for the detection of veterinary pathogens?

PERFORMANCE : ADIAVET™ processes are validated from sample to identification

FLEXIBILITY : kits include all the different steps of the analysis but remain fully opened for different extraction methods and different thermocyclers

OPTIMIZED WORKFLOW : global extraction method and/or PCR amplification for simultaneous testing of several pathogens

DNA/RNA extraction step

In order to achieve very good performances on a wide range of samples, Adiagene always recommends and validates many different DNA/RNA global extraction methods. It is very useful to stay flexible without decreasing any performances (sensitivity, inhibition rate).

Visit the options & accessories tab to find out more on our Adiapure offer for DNA/RNA extraction.

PCR amplification step

ADIAVETTM PCR kits are ready-to-use  to reduce risk of mistakes during PCR setup. They are easy-to-use, with only 2 pipetting steps :

  • MasterMix distribution
  • DNA/RNA transfer

They include all components  for DNA amplification. Real Time PCR detection system is based on hydrolysis probe to get a better specificity and to allow multiplexing.

An internal control is included inside each kit to check the quality and stability of the samples, the performances of nucleic acids extraction/purification and the absence of inhibition of the PCR amplification. Positive control(s) included inside each kit are also here to check capability and sensitivity of the kit to detect targeted pathogen(s).

PCR amplification cycles are harmonized as much as possible to give possibility of a multidetection system on the same run and the same thermocycler. Most of Real Time thermocyclers used on the market have been validated by Adiagene and can be used without any risk of performances decrease.

 

BTV – BLUETONGUE VIRUS PCR KIT

ADIAVET™ BTV REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Bluetongue virus (all serotypes)

FAM (BTV)

VIC (internal control)

 

Blood, tissues

 

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ BTV TYPE 8 REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Bluetongue virus, serotype 8

FAM (BTV serotype 1)

VIC (internal control)

 

Blood, tissues

 

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ BTV TYPE 1 REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Bluetongue virus, serotype 1

FAM (BTV serotype 1)

VIC (internal control)

 

Blood, tissues

 

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ BTV TYPE 6 REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Bluetongue virus, serotype 6

FAM (BTV serotype 6)

VIC (internal control)

 

Blood, tissues

 

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ BTV Group + TYPE 8 REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Bluetongue virus, all serotypes

Bluetongue virus, serotype 8

FAM (BTV all serotypes)

VIC (BTV serotype 8)

Cy5 (internal control)

 

 

Blood, tissues

 

 

-20°C +/-5°C

 

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ BTV Group + TYPE 1 REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Bluetongue virus,
all serotypes

Bluetongue virus, serotype 1

FAM (BTV all serotypes)

Cy5 (BTV serotype 1)

VIC (internal control)

 

 

Blood, tissues

 

 

-20°C +/-5°C

 

Endogenous (GAPDH), validates extraction and amplification

 

BVD/BD – BOVINE VIRAL DIARRHEA PCR KIT

 

ADIAVET™ BVD REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

BVDV (Bovine Viral Diarrhea Virus) and BDV (Border Disease Virus)

FAM (BVD and BD viruses)

VIC (internal control)

Blood, serum, tissues (lung, spleen…), milk, ear tag

 

 

-20°C +/-5°C

Endogenous (RNASE P), validates extraction and amplification

 

 

CHLAMYDOPHILA ABORTUS PCR KIT

ADIAVET™   CHLAM.A. REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Chlamydophila abortus

FAM (C. abortus)

VIC (Internal control)

 

Tissues (placenta), swabs, milk

 

+2/8°C

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ CHLAMCOX CY5 REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Chlamydophila abortus

Coxiella burnetii

FAM (C. abortus)

VIC (C. burnetii)

Cy5 (internal control)

 

Tissues (placenta), swabs, milk

 

 

+2/8°C

Endogenous (GAPDH), validates extraction and amplification

COXIELLA BURNETII PCR KIT

ADIAVET™ COX REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Coxiella burnetii

 

FAM (C. burnetii)

VIC (Internal control)

 

Tissues (placenta), swabs, milk, faeces

 

 

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

ADIAVET™ CHLAMCOX CY5 REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

Chlamydophila abortus

Coxiella burnetii

FAM (C. abortus)

VIC (C. burnetii)

Cy5 (internal control)

Tissues (placenta), swabs, milk

 

+2/8°C

Endogenous (GAPDH), validates extraction and amplification

 

LEPTOSPIRA PCR KIT

ADIAVET™ LEPTO REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Leptospira

FAM (Leptospira)

VIC (internal control)

Blood, urine, tissues (kidney, liver, spleen…)

 

+2/8°C

Exogenous, validates amplification

 

MYCOBACTERIUM AVIUM SUBSP PARATUBERCULOSIS PCR KITS

ADIAVET™ PARATB REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Mycobacterium avium subsp. paratuberculosis

FAM (M. paratuberculosis)

VIC (Internal control)

 

Faeces, bacterial culture, milk, tissues

 

 

-20°C +/-5°C

Exogenous, validates extraction and/or amplification steps

SBV – SCHMALLENBERG VIRUS PCR DETECTION KIT

ADIAVET™ SCHMALLENBERG VIRUS REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

SBV - Schmallenberg virus

 

FAM (SBV)

VIC (Internal control)

 

Tissues (brain, spleen), whole blood, serum

 

 

-20°C +/-5°C

Endogenous (GAPDH), validates extraction and amplification

TOXOPLASMA GONDII PCR KIT

ADIAVET™ TOXO REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Toxoplasma gondii

 

FAM (T. gondii)

VIC (internal control)

 

Tissues (heart, placenta, brain…)

 

+2/8°C

Endogenous, validates extraction and amplification

 

Today DNA and RNA extracts usable with ADIAVETTM kits can be prepared with :

  • Different technologies of purification : silica membrane, magnetic beads, ultra-filtration
  • Different automated system : centrifugation, vacuum, magnetic
  • For different throughputs : from individual analysis to 96 samples extraction per run

For some specific applications Adiagene proposes its own extraction method with the AdiapureTM range in combination with ADIAVETTM PCR kits:

  • AdiapureTM milk kit: for DNA extraction of Mycobacterium avium subsp. paratuberculosis from dairy products
  • AdiapureTM TLB buffer: single lysis buffer for RNA extraction of BVD virus from ear notches samples

Samples preparation steps (individual or pool) are developed and validated to be adapted to different epidemiological situations : diagnostic, individual analysis, screening with pool of samples or environmental samples. The harmonization of DNA/RNA extraction methods is sought as much as possible to combine different analysis all together. 

Pioneering diagnostics