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ADIAVET™ Avian

ADIAVET™ Real time PCR diagnostic kits for avian species diseases

  • Easy-to-use
  • Complete solution
  • Validated processes
     
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ADIAVET™ diagnostic kits for avian species

 

By providing quality microbial identification solutions for animal species, we hope to reduce the risk of economic diseases, affecting farmers and breeders. As we also prevent the risk of zoonotic diseases, we contribute to ensuring public health and safety.

Our range of ADIAVET™ ready-to-use PCR kits were designed to make identification of avian diseases easier and faster for you.

From sample preparation…

To perform quality identification, we recommend several DNA/RNA extraction methods adapted to your needs.

For avian species:

SILICA MEMBRANE PURIFICATION KITS

DNA EXTRACTION & PURIFICATION KIT ADIAPURE

…to real time PCR kits : a complete solution for avian species

 

Our large range of solutions are designed to perform reliable diagnosis of diseases causing among avian species :

  • Respiratory troubles

  • Mobility disorders

  • Nervous system troubles

 

ADIAVET™ kits for avian pathologies detection:

  • IV - Influenza Virus PCR detection kit for avian host 

  • MAREK - PCR kit for the detection of the MAREK Virus

  • MYCO AV - Avian Mycoplasma PCR Detection Kit 

  • ORT - Ornithobacterium rhinotracheale Detection Kit

  • WNV – West Nile virus PCR Detection Kit

Find out more details on the ADIAVET™ range for avian species on the technical details tab.

 

Why choose ADIAVET™ for the detection of veterinary pathogens?

PERFORMANCE : ADIAVET™ processes are validated from sample to identification

FLEXIBILITY : kits include all the different steps of the analysis but remain fully opened for different extraction methods and different thermocyclers

OPTIMIZED WORKFLOW : global extraction method and/or PCR amplification for simultaneous testing of several pathogens 

DNA/RNA extraction step

In order to achieve very good performances on a wide range of samples, Adiagene always recommends and validates many different DNA/RNA global extraction methods. It is very useful to stay flexible without decreasing any performances (sensitivity, inhibition rate).

Visit the options & accessories tab to find out more on our Adiapure offer for DNA/RNA extraction.

PCR amplification step

AdiavetTM PCR kits are ready-to-use  to reduce risk of mistakes during PCR setup. They are easy-to-use, with only 2 pipetting steps :

  • MasterMix distribution
  • DNA/RNA transfer

They include all components  for DNA amplification. Real Time PCR detection system is based on hydrolysis probe to get a better specificity and to allow multiplexing.

An internal control is included inside each kit to check the quality and stability of the samples, the performances of nucleic acids extraction/purification and the absence of inhibition of the PCR amplification. Positive control(s) included inside each kit are also here to check capability and sensitivity of the kit to detect targeted pathogen(s).

PCR amplification cycles are harmonized as much as possible to give possibility of a multidetection system on the same run and the same thermocycler. Most of Real Time thermocyclers used on the market have been validated by Adiagene and can be used without any risk of performances decrease.

 

AIV - INFLUENZA VIRUS TYPE A PCR KIT FOR AVIAN HOST

ADIAVET™ AIV  REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Avian Influenza A Virus (AIV)

FAM (AIV)

VIC (internal control)

 

Swabs, tissues, faeces

 

-20°C +/-5°C

Endogenous, validates extraction and amplification

 

KIT FOR THE DETECTION OF THE MAREK VIRUS

ADIAVET™ MAREK REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Marek virus

FAM (Marek virus)

VIC (Internal Control)

 

Environmental air samples

-20°C +/-5°C

Exogenous, control extraction and amplification steps

AVIAN MYCOPLASMA PCR KITS

ADIAVET™  MYCO AV REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

M. gallisepticum

M. synoviae

FAM (M. synoviae)

Cy5 (M. gallisepticum)

VIC (Internal control)

 

Swabs, bacterial culture, drag swabs

 

+2/8°C

Exogenous, validates amplification

ADIAVET™ MG REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

M. gallisepticum

FAM (M. gallisepticum)

VIC (Internal control)

 

Swabs, bacterial culture, drag swabs

 

+2/8°C

Exogenous, validates amplification

ADIAVET™ MS REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

M. synoviae

FAM (M. synoviae)

VIC (Internal control)

Swabs, bacterial culture, drag swabs

 

+2/8°C

Exogenous, validates amplification

ADIAVET™ MYCO-AV

Pathogen

Dyes

Samples

Storage

Internal Control

M. gallisepticum

M. synoviae

M. meleagridis

 

Conventional PCR: Single-well multiplex kit

 

Swabs, bacterial culture, drag swabs

 

+2/8°C

Exogenous, validates amplification

 

ORNITHOBACTERIUM RHINOTRACHEALE PCR KIT

ADIAVET™ ORT REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

Ornithobacterium rhinotracheale

 

FAM (ORT) VIC (Internal Control)

 

swab, drag swab

 

-20°C +/-5°C

Endogenous (GAPDH), validates amplification

WEST NILE VIRUS PCR DETECTION KIT

ADIAVET™ WEST-NILE REAL TIME

Pathogen

Dyes

Samples

Storage

Internal Control

 

West-Nile Virus - WNV

 

FAM (WNV)

VIC (internal control)

Cerebrospinal liquid, tissues (brain, lung…), swabs

 

-20°C +/-5°C

Endogenous (beta actin), validates extraction and amplification

 

Today DNA and RNA extracts usable with ADIAVETTM kits can be prepared with :

  • Different technologies of purification : silica membrane, magnetic beads, ultra-filtration
  • Different automated system : centrifugation, vacuum, magnetic
  • For different throughputs : from individual analysis to 96 samples extraction per run

For some specific applications Adiagene proposes its own extraction method with the AdiapureTM range in combination with ADIAVETTM PCR kits :

  • AdiapureTM purification kit: for DNA extraction of Avian mycoplasma and Ornithobacterium rhinotracheale from swabs

Pioneering diagnostics